Naphthol AS-BI

Naphthol AS-BI is a functionally specialized naphthol derivative developed for applications that demand high molecular selectivity and signal precision. Structurally based on 2-hydroxy-3-naphthylformanilide, the compound is engineered to exhibit controlled reactivity, making it suitable for both enzyme-responsive diagnostic systems and high-value azo dyeing processes. The product is supplied as a fine crystalline powder with stable composition and reproducible performance characteristics.

In biomedical and life-science applications, the phosphate ester form of Naphthol AS-BI is widely recognized as a reference substrate for phosphatase-related analyses. Upon enzymatic action by alkaline or acid phosphatase, the phosphate group is selectively cleaved, releasing free Naphthol AS-BI. The liberated compound can either produce intense fluorescence or participate in a coupling reaction with diazonium salts to form a sharply localized, insoluble color deposit. This dual signal capability enables accurate visualization of enzyme activity at the cellular and subcellular level. As a result, AS-BI phosphate is extensively used in histochemical and cytochemical staining protocols, including Tartrate-Resistant Acid Phosphatase (TRAP) assays for osteoclast identification, as well as diagnostic investigations related to hematological and oncological research.

In parallel, Naphthol AS-BI also functions as a high-selectivity coupling component in azo dye systems for specialty textiles. The activated naphthol hydroxyl group readily reacts with suitable diazonium salts, forming insoluble azo pigments directly within fiber structures. This in-fiber pigment generation produces clean, well-defined shades with excellent resistance to washing, rubbing, and light exposure. Due to its controlled coupling behavior and shade clarity, AS-BI is particularly suited for premium substrates such as silk, medical textiles, and technical fabrics where color accuracy and durability are critical.

Because of its use in sensitive analytical and industrial environments, Naphthol AS-BI requires careful handling. The material should be stored in sealed, light-resistant containers under dry and cool conditions to preserve both its enzymatic responsiveness and coupling performance. Each batch is manufactured and quality-controlled to ensure consistency, supporting reliable results across diagnostic assays and specialized dyeing applications.

Naphthol AS-BI represents a purpose-driven chemical solution that integrates molecular precision with practical performance, supporting advanced research and high-standard textile coloration within a single, well-defined product.

Handling and storage of this product should be carried out with appropriate care to minimize potential risks. Prolonged or unnecessary exposure should be avoided, and all handling operations should be conducted cautiously in accordance with standard chemical safety practices. Exposure to the substance should be kept as low as reasonably achievable, and inhalation of dust or vapor must be strictly avoided. Adequate ventilation is essential during use, and operations should preferably be performed in a chemical fume hood. Safety showers and eye-wash facilities should be readily available in the working area. Direct contact with eyes, skin, or clothing should be prevented, and suitable personal protective equipment should be worn at all times. Containers must be kept tightly closed when not in use. The product should be stored in a cool, dry, and well-ventilated location. It should be noted that, to the best of current knowledge, the health hazards of this product have not been fully investigated, and it is supplied solely for research and development purposes.

Shandong Inno-Chem CO., LTD. founded in 2010, which is a comprehensive industrial group specializing in the production and operation of a series of dyes, including disperse, liquid disperse, solvent, reactive, liquid reactive, vat, direct, acid and cationic dyes, dye intermediates, textile printing and dyeing auxiliaries, sodium hydrosulfite and other chemicals, as well as TPU films. We are one of the leading dye and pigment manufacturers and exporters in China.